White Blood, Red Blood

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Sunday, September 27, 2009

Blood Separation

We can separate blood by gravity like on the test tube. If a test tube of blood is left to stand for half an hour, the blood separates into three layers as the denser components sink to the bottom of the tube and fluid remains at the top. The tree parts of blood differ with the color seen, the top with straw colored, and the middle with white color and at the bottom with old red color.

The straw-colored fluid that forms the top layer is called plasma and forms about 60% of blood. The middle white layer is composed of white blood cells (WBCs) and platelets, and the bottom red layer is the red blood cells (RBCs). These bottom two layers of cells form about 40% of the blood.

Plasma is mainly water, but it also contains many important substances such as proteins (albumin, clotting factors, antibodies, enzymes, and hormones), sugars (glucose), and fat particles.

All of the cells found in the blood are produced from bone marrow. They begin their life as stem cells, and they mature into three main types of cells— RBCs, WBCs, and platelets. In turn, there are three types of WBC—lymphocytes, monocytes, and granulocytes—and three main types of granulocytes (neutrophils, eosinophils, and basophils).

This separate part of blood can be separated by centrifuge method. A sample of blood can be further separated into its individual components by spinning the sample in a centrifuge. The principle work of using spinning rotation by centrifuge cause the denser compound of blood is sink, and further processing enables the isolation of a particular protein or the isolation of a particular type of blood cell. With the use of this method, antibodies and clotting factors can be harvested from the plasma to treat immune deficiencies and bleeding disorders, respectively. Likewise, RBCs can be harvested for blood transfusion.